| Congresso Brasileiro de Microbiologia 2023 | Resumo: 976-1 | ||||
Resumo:The coffee industry is of great economic relevance worldwide. In Brazil coffee is the fourth agricultural product most produced in the country. However, with such a great production, it is estimated that about the same produced amount in coffee resulted in waste production, since the seed, part of the economic value of the fruit, makes up 50% of its total weight. The need for treatment and disposal of waste from coffee processing has driven studies in order not only to treat but also give value to it, since the coffee plant biomass is rich in relevant by-products for the industry. In addition to the benefits of biotransformation, a new class of green solvents has shown great potential to aid the extraction of relevant compounds from the coffee husk. The goal of the present study is to evaluate the biotransformation of the residual plant biomass of the coffee industry, aiming at the extraction of compounds with industrial relevance, thus attributing value to the residue. The microorganisms used in this work were selected based on their ligninolytic potential, including an endophytic fungus and an actinomycete isolate. It was adopted deep eutectic solvents as green carriers for the microbial transformation of the biomass. The cultures were established using mineral medium ISP9 added by 1% coffee biomass and 1% deep eutectic solvents. The experiments were monitored by chromatographic analysis using High Performance Liquid Chromatography Coupled to Mass Spectrometry (HPLC-MS/MS). The results allowed us to identify the occurrence of the biotransformation of lignin monomers by the selected microorganisms, allowing the characterization of polyphenolic compounds, such as phenylpropanoids. Therefore, it was possible to observe that the biotransformation resulted in the extraction of interesting compounds for the industry. Phenolic compounds were also identified in other studies in the literature, with residues from coffee processing as plant biomass, corroborating with the findings in the present study. Palavras-chave: bioactives, biotransformation, deep eutectic solvents, microorganisms, plant biomass Agência de fomento:FAPESP | |||||